over agar antibiotic plating
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Addgene: Protocol - Over-Agar Antibiotic Plating
Plate 50 µL of transformed E. coli/rescue media suspension onto the agar and gently spread over the surface until the liquid is mostly absorbed. The spreading of cells can be done in the same way as the antibiotic, using either a bent micropipette tip or other cell spreading device that fits the plate.
Pouring LB Agar Plates - addgene
Sep 14, 2016· Swirl the agar bottle to ensure even distribution of the antibiotic throughout the agar. Open one plate at a time next to the flame and begin pouring. Measure your desired amount of agar with a pipete for the first plate to get a good idea of what that volume looks like in your particular plate.
Over-Agar Antibiotic Plating
Oct 30, 2017· Luckily, there is a relatively quick way to add antibiotic to your plates in a pinch. Watch this video to learn how to perform over-agar antibiotic plating. For the full written Over-Agar .
How to Make the Perfect Agar Plate Every Time - Bitesize Bio
Spread the antibiotic solution after the agar on the plate get solidified? Hi, I am used to preparing LB plates with antibiotic solution added to liquid medium and pour the mix into the plate..